pak4 p 21 (Santa Cruz Biotechnology)
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pak4 p 21
Pak4 P 21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pak4+p+21/p21/pmc10149936-19-3-14
Average 96 stars, based on 5645 article reviews
Pak4 P 21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pak4+p+21/p21/pmc10149936-19-3-14
Average 96 stars, based on 5645 article reviews
pak4 p 21 - by Bioz Stars,
2026-09
96/100 stars
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1) Product Images from "Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth"
Article Title: Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth
Journal: Frontiers in Physiology
doi: 10.3389/fphys.2023.1147572
Figure Legend Snippet: Effect of two PAK4 inhibitors, PF-3758309 or LCH-7749944, on the ability of CCK-8 (0.3 and 100 nM) or TPA (1 µM) to alter activation of cofilin. Isolated pancreatic acini were incubated in the absence or presence of PF-3758309 (0.1 nM) or LCH-7749944 (30 µM) for 3 h and then incubated with no additions (control), CCK-8 (0.3 and 100 nM) for 3 min or TPA (1 µM) for 5 min, and then lysed. Western blots were analyzed using anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors; N.S. , No significant.
Techniques Used: CCK-8 Assay, Activation Assay, Isolation, Incubation, Control, Western Blot
Figure Legend Snippet: Schematic diagram of signaling cascade for activation of cofilin in pancreatic acinar cells. In rat pancreatic acinar cells, maximal activation of cofilin by cholecystokinin (CCK)-8 requires activation of PKC, which mediates both Src family of kinase (SFK) and protein kinase D (PKD) activation resulting in PAK4 activation; requires activation of ROCK, which is mediated by Rho; requires activation of JNK, mediated by activation of this MAPK pathway; and requires activation of serine protein phosphatases (PP2A), which are a substrate of PKC. Cofilin activation is important for CCK-stimulated enzyme secretion as well as ERK1/2 activation which has been shown to mediate growth. Squares represent signaling pathways shown to be involved in cofilin activation in this study.
Techniques Used: Activation Assay, CCK-8 Assay, Protein-Protein interactions
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